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Biotium
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Thermo Fisher
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Image Search Results
Journal: Journal of Translational Medicine
Article Title: Excessive proliferation and impaired function of primitive hematopoietic cells in bone marrow due to senescence post chemotherapy in a T cell acute lymphoblastic leukemia model
doi: 10.1186/s12967-015-0543-8
Figure Lengend Snippet: Apoptosis has little effect on changes of LK + S − /LK + S + hematopoietic cells since the recovery phase. a Gating strategy for apoptosis using 7-AAD and Annexin-V staining. The cellular uptake of these dyes discriminated cells in Alive (7-AAD low Annexin-V low ), Necrosis (Annexin-V low 7-AAD high ) and Apoptosis (Annexin-V high 7-AAD low ). b – e Percentages of viable CD45.2 + LK + S − cells ( b ), apoptotic CD45.2 + LK + S − cells ( c ), viable CD45.2 + LK + S + cells ( d ), apoptotic CD45.2 + LK + S + cells ( e ) in the BM of normal control (shown as N) and the 1-day treated leukemic mice on different days (n = 4–5).
Article Snippet: For apoptosis assays, the staining was performed in the staining buffer with FITC conjugated Annexin-V and
Techniques: Staining